Table 1.
Strains and plasmids used in this study
| Strain or plasmid | Relevant characteristics | Source or reference |
|---|---|---|
| E. coli strains | ||
| TOP 10 | F−mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74 recA1 araD139 Δ(ara-leu)7697 galUgalKrpsL (StrR) endA1 nupG | Invitrogen |
| S17-1 | RP4 tra region, mobilizer strain recA derivative of MM294A with RP4-2 (Tc : : Mu : : Km : : FabF2/F17) integrated into the chromosome | Simon et al. (1983) |
| DH5α | F−φ80lacZΔM15 Δ(lacZYA-argF)U169 recA1 endA1 hsdR17( ,  ) phoAsupE44 thi-1gyrA96 relA1 λ−
|
Invitrogen |
| R. leguminosarum strains | ||
| 3841 | Biovar viciae, JB300 derivative, Smr | Johnston & Beringer (1975) |
| 38EV27 | 3841 fabF2, fabF1 TGN mutant, Smr, Nmr, Gmr | This study |
| 38EV51 | 3841 fabF2/F1 mutant with functional fabF1 expressed on plasmid pEV51, Smr, Nmr, Gmr, Tcr | This study |
| 38EV28 | 3841 fabF1 mutant, Smr, Gmr | This study |
| Plasmids | ||
| pTGN | Tn5 derivative, Gmr, Ampr promoterless Nmr, gfp | Tang et al. (1999) |
| pCR2.1 TOPO | TOPO TA cloning vector, 4.0 kb, Kmr, Apr | Invitrogen |
| pDG71 | Broad-host-range derivative of pHC41. Constitutively expressed tryptophan promoter and gfp(mut3), Tcr | Bringhurst et al. (2001) |
| pJQ200SK+ | Suicide vector, P15aori, mob, sacB, Gmr | Quandt & Hynes (1993) |
| pFUS1 | pMP220 derivative with promoterless gusA, Tcr | Reeve et al. (1999) |
| pFUS1P | pFUS1, par cassette inserted from pRR71-H as HindIII fragment | Yost et al. (2004) |
| pCS115 | pWKR56 carrying HindIII fragment from pRIA76, Tcr | Colonna-Romano et al. (1990) |
| pEV35 | pCR2.1 TOPO with 1330 bp PCR fragment containing fabF1, Ampr, Kmr | This study |
| pEV51 | 1330 bp fabF1 fragment cloned from pEV35 into pDG71 with BamHI, Tcr | This study |
| pEV26 | pCR2.1 TOPO with 456 bp PCR fragment containing internal fragment of fabF1, Ampr, Kmr | This study |
| pEV28 | 456 bp fabF1 fragment cloned from pEV26 into pJQ200SK+ using ApaI and BamHI, Gmr | This study |
| pNdvB | pFUS1P, ndvB : : gusA, Tcr | This study |
| pAVP | pFUS1, flaA : : gusA, Tcr | Michael Hynes |
| pVNVP | pFUS1P, visN : : gusA, Tcr, par stabilized | Michael Hynes |
| pSVP SUM | pFUS1P, rem : : gusA, Tcr, par stabilized | Michael Hynes |
| pCGR | pRK7813, mcpC : : gusA, Nmr, Tcr | Yost et al. (2004) |
| pDGRP | pFUS1P, mcpD : : gusA, Tcr, par stabilized | Yost et al. (2004) |