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. Author manuscript; available in PMC: 2011 Jan 1.
Published in final edited form as: Cell Microbiol. 2009 Aug 27;12(1):31–41. doi: 10.1111/j.1462-5822.2009.01376.x

Figure 4. EspF is translocated and functional in cells infected with EPECΔnleA.

Figure 4

A. Translocation assay: HeLa cells were infected with the strains indicated and subjected to subcellular fractionation. Fractions were probed with antisera against EspF, Tir, and Calreticulin. B. HeLa cells seeded onto glass coverslips were infected for 90 minutes with wt EPEC or EPEC ΔnleA , or left uninfected (ui). Cells were stained with DAPI (left panels, blue in the overlay) and anti-SNX9 antibodies (center panels, green in the overlay). Areas demonstrating changes in SNX9 localization after wt EPEC or ΔnleA infection as compared to uninfected are enlarged in the overlay panels.