Figure 3. Specificity of Hpca antibody.
2 μg of each: frequenin (FQ), GCAP1, GCAP2, Hpca, neurocalcin δ (Ncal δ), and S100β were separated by SDS-15%PAGE. One gel was stained with Coomassie blue (STAINING) and another gel containing the same set of proteins was transferred to PVDF membranes and probed with anti Hpca antibody (WESTERN). The band corresponding to Hpca is indicated by an arrow. Molecular size markers are given alongside.