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. Author manuscript; available in PMC: 2010 Apr 6.
Published in final edited form as: Mol Cell Biochem. 2009 Jan 23;325(1-2):1–14. doi: 10.1007/s11010-008-0015-z

Figure 8. Hpca-modulated ONE-GC system in the olfactory neuroepithelium.

Figure 8

Particulate fractions of the olfactory neuroepithelium were isolated from the wild type and Hpca−/− mice in the presence of 100 μM Ca2+. Membranes were assayed for the guanylate cyclase activity in the presence of 1 mM EGTA or 100 μM Ca2+ (A), in the presence of 100 μM Ca2+ and Hpca antibody (1: 100 dilution) (B), and in the presence of 100 μM Ca2+ and indicated concentrations of myr-Hpca (C). Cyclic GMP formed was measured by radioimmunoassay. The experiment was done in triplicate and repeated two times with separate membrane preparations. The results presented (mean ± SE) are from one experiment.