FIG. 4.

Detection of CD34⁺ microvasculature: brain tissues from indicated regions (small images, left) were immunochemically stained for CD34. (A and B) Representative of CD34 stained tissue from the boundary of the injured cerebral tissue, showing CD34⁺ endothelial-like lumen structures on day 3 after TBI. (C and D) Representative OF CD34 staining of tissue from the injured tissue, also showing CD34⁺ endothelial-like lumen structures, but only at approximately day 14 after TBI. Arrows indicated the vascular lumen-like structure. The images were representative from CD34-stained tissue sections. Bar, 30 μm (A and B) or 50 μm (C and D).