Table 3.
List of the differentially expressed protein spots in 2DE of HIV-positive and healthy donors identified by ESI-MS or both ESI-MS and MALDI-TOF/TOF.
| spota | Accession NO.b | Protein description | MWc | pI | Scored | Cov.e | Abundance HIV/NOR | Functionf | Locationg |
|---|---|---|---|---|---|---|---|---|---|
| 1 | KPYM_HUMAN | Pyruvate kinase isozymes M1/M2 - Homo sapiens (Human) | 58470 | 7.96 | 173 | 15% | ↑ | enzyme | Cytosol |
| 2 | TLN1_HUMAN | Talin-1 - Homo sapiens (Human) | 271766 | 5.77 | 44 | 2% | ↑ | cell-cell junction | Cell membrane; Cell projection; Cytoplasm; Cytoskeleton; Membrane. |
| 3 | LDHB_HUMAN | L-lactate dehydrogenase B chain - Homo sapiens (Human) | 36900 | 5.71 | 316 | 30% | ↑ | enzyme | Cytoplasm. |
| 4, 14, 15 | CAP1_HUMAN | Adenylyl cyclase-associated protein 1 - Homo sapiens (Human) | 52222 | 8.27 | 129 | 16% | ↑ | actin binding | Cell membrane; PM |
| 5, 13, 16, 19 | ENOA_HUMAN | Alpha-enolase - Homo sapiens (Human) | 47481 | 7.01 | 217 | 34% | ↑ | Enzyme | Cell membrane; Cytoplasm; Membrane; Nucleus. |
| 6 | EHD3_HUMAN | EH domain-containing protein 3 - Homo sapiens (Human) | 61971 | 6.06 | 285 | 36% | ↑ | Binding/transport | Cell membrane; Endosome. Membrane |
| 7 | COR1C_HUMAN | Coronin-1C - Homo sapiens (Human) | 53899 | 6.65 | 253 | 18% | ↑ | Actin-binding/transport | actin cytoskeleton |
| 8 | ST1A3_HUMAN | Sulfotransferase 1A3/1A4 - Homo sapiens (Human) | 34288 | 5.68 | 85 | 23% | ↑ | Actin-binding/transport | Cytoplasm |
| 9 | FLNA_HUMAN | Filamin-A - Homo sapiens (Human) | 283301 | 5.7 | 172 | 9% | ↑ | Actin-binding | Cytoplasm; Cytoskeleton; plasma membrane. |
| 10 | VINC_HUMAN | Vinculin - Homo sapiens (Human) | 124292 | 5.5 | 755 | 26% | ↑ | actin binding | Cytoplasm; cytoskeleton; Cell junction; adherens junction; Cell membrane; Peripheral membrane protein; |
| 11 | IGKC_HUMAN | Ig kappa chain C region - Homo sapiens (Human) | 11773 | 5.58 | 101 | 80% | ↑ | immuno | extracellular region |
| 12 | GNB1_HUMAN | Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 - Homo sapiens (Human) | 38151 | 5.6 | 69 | 31% | ↓ | transducer | |
| 17, 18, 20 | ACTB_HUMAN | Actin, cytoplasmic 1 | 42052 | 5.29 | 119 | 29% | ↑ | ATP binding | Cytoplasm; cytoskeleton. |
aSpot no. is the unique number which refers to the labels in Figure 1. Protein spots identified by ESI and MALDI were highlighted by bold and italic.
bAccession no. is the MASCOT results of ESI-Ion trap searched from the SWISS-PROT database.
c Molecular weight predicted from database.
d Mascot score was selected identified by HCT.
e Sequence coverage (%) means the number of amino acids spanned by the assigned peptides divided by the sequence length.
fProtein function from SWISS-PROT database
g Protein location from SWISS-PROT database