Figure 4.

Conversion of the halothane-induced inhibition into potentiation by a single substitution in the S6 PVPV motif of the Shaw2 channel. (A) Whole-oocyte Shaw2 P410A currents evoked by step depolarizations from −100 to +60 mV delivered at 30 s intervals. Several superimposed traces are shown before adding halothane, in the presence of 0.46 or 1 mM halothane and after washout (C, H and W respectively). (B) Bar graph summarizing the mean % change induced by two concentrations of halothane (0.46 or 1 mM). Negative and positive changes correspond to inhibition and potentiation respectively. From left to right, the number of independent determinations was: 6, 8, 8 and 9.