Skip to main content
. 2010 Jan-Mar;4(1):45–52. doi: 10.4161/pri.4.1.11074

Figure 2.

Figure 2

Polymers of Q70 and QY76 cause polymerization of the plasmid-encoded Sup35NM-2HAprotein in Δrnq1 cells. (A) The plasmid pRS315-SUP35C of the strain 74-D694ΔS35 [psi−][PIN+] was shuffled for either pQ70-Sup35MC or pQY76-Sup35MC. Then, the RNQ1 gene was deleted. Polymers of Q70 and QY76 were revealed using SDD-AGE and western blotting with polyclonal anti-Sup35NM antibody. (B) These strains were transformed with a multicopy plasmid, encoding Sup35NM-2HA. Appearance of Sup35NM-2HApolymers was tested using SDD-AGE and western blotting with monoclonal anti-HA antibody. PolyQ, the Q70 or QY76 protein; −70, −76, transformants that have lost the plasmid encoding indicated polyQ protein. Sup35NM-2HA, − or +, absence or presence of the plasmid encoding this protein. RNQ1, +, chromosomal wild type allele, Δ, disruption allele.