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Polymers of Q70 and QY76 cause aggregation of Sup35NM-GFP in cells lacking [PIN+]. Multicopy plasmids encoding Q70 or QY76 were introduced into 74-D694 [psi−][PIN+] cells and then RNQ1 was deleted to remove [PIN+]. After this, the plasmid encoding Sup35NM-GFP was introduced. About 25% of transformed cells contained fluorescent dots. The same strain, but without the pQ70-Sup35MCand pQY76-Sup35MCplasmids was used as a control.
