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Co-appearance of SDS-resistant polymers of Q70, Rnq1 and Pub1. Cells of the 74-D694ΔS35 [psi−][pin−-] strain were transformed with the pQ70-SUP35MC plasmid. Samples were taken at 30 (lane 1) and 100 (lane 2) cell generations after transformation. Then the pQ70-SUP35MC plasmid was replaced with pRS315-SUP35C (lane 3). The samples were analyzed by SDD-AGE and western blotting. The proteins indicated below were revealed using antibodies to Sup35NM, Rnq1 and Pub1, respectively.
