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. 2010 Apr 9;86(4):632–638. doi: 10.1016/j.ajhg.2010.02.025

Figure 3.

Figure 3

Mutant K74 Protein Disrupts Endogenous KIF Formation in PtK2 Cells, and K74 Colocalizes with LPAR6 in Huxley's Layer of Human Hair Follicles

(A–C) Ectopically expressed wild-type (Wt) K74 protein forms a KIF network via heterodimerization with endogenous K18 protein.

(D–F) The p.Asn148Lys mutant (Mut) K74 protein causes a collapse of the endogenous KIF network around the nucleus.

(G–I) Double immunostainings with guinea pig polyclonal anti-K74 (1:2,000) (G) and rabbit polyclonal anti-P2Y5 (LPAR6; 1:200; MBL International) (H) antibodies show that both K74 and LPAR6 proteins are coexpressed in Huxley's (Hux) layer of the IRS of human hair follicles from a normal control individual (I), whereas only LPAR6 is expressed in Henle's (He) layer of the IRS (I).

The right panels are merged images, and counterstaining with DAPI is shown in blue (C, F, and I). Scale bars represent 20 μm (A) and 100 μm (G).