Table I.

Effect of Type and Concentration of Cryoprotectant after Freeze–Thaw Study

S. no.	Cryoprotectant	Concentration% (w/v)	Initial size (Si), nm (Avg ± std error), (n = 3)	Final size (Sf), nm (Avg ± std error), (n = 3)	Sf/Si ratio
1	Trehalose	5	405.2 ± 1.27	2,956.2 ± 0.68	7.30
		10	405.2 ± 1.27	1,248.3 ± 2.03	3.08
		15	405.2 ± 1.27	642 ± 2.38	1.58
		20	405.2 ± 1.27	443.5 ± 2.97	1.09
2	Fructose	5	405.2 ± 1.27	>3,000	>7.4
		10	405.2 ± 1.27	>3,000	>7.4
		15	405.2 ± 1.27	2,431.9 ± 0.96	6.00
		20	405.2 ± 1.27	977.9 ± 1.48	2.41