Table II.

Effect of Freezing Temperature and Concentration of Nanoparticles on Particle Size during Freeze–Thaw Study

Concentration of nanoparticles (mg/ml)	Initial size (nm)	Particle size (nm) after freeze–thaw study
		Trehalose (20% w/v)		Fructose (20% w/v)
		−40°C	−70°C	−40°C	−70°C
8	382.5 ± 2.01	386.9 ± 2.24	394.6 ± 2.31	746.1 ± 3.13	774 ± 3.52
16	373.5 ± 2.45	403.6 ± 2.81	389.4 ± 3.11	758.3 ± 2.76	737.6 ± 2.63
32	405.2 ± 1.27	443.5 ± 1.75	436.5 ± 3.28	977.9 ± 1.89	861.2 ± 2.28
64	411.6 ± 1.96	971.5 ± 2.59	991.5 ± 1.86	1,203.5 ± 1.67	1,289.5 ± 2.05