Table 2.
Effect of priming in water (hydropriming) or in polyethylene glycol (PEG) 8000 (osmopriming) for different durations on germination of Titan lettuce seeds at 20 and 35°C
| Type of priming | Imbibition temperature (°C) | Germination (%) | ||||
|---|---|---|---|---|---|---|
| Priming duration at 9°C (h) | ||||||
| 0 | 6 | 12 | 24 | 48 | ||
| H20 | 20 | 98 ± 1 | 96 ± 2 | 97 ± 1 | 96 ± 2 | 100 |
| 35 | 0 | 0 | 12 ± 2 | 68 ± 6 | 75 ± 12 | |
| PEG | 20 | 97 ± 1 | 93 ± 1 | 100 | 100 | 96 ± 2 |
| 35 | 0 | 0 | 1 ± 1 | 40 ± 4 | 88 ± 6 | |
After priming for the indicated durations at 9°C, seeds were rapidly dried (2 h at 32°C and 30% RH) and equilibrated at 9°C and 30% RH for 2 days. Subsequently, the seeds were imbibed at 20 and 35°C and radicle emergence was scored after 48 h. Three replicates of 25 seeds were tested for each treatment; means and standard errors are indicated