Figure 3.

(a) Polymerase ELISA. Shown are the hairpin substrate and activities of round 1 clones (15, A23, 55, AH12) and round 2 clones (9, 10, E10, 23). Clones were chosen i.a. on the their ability to incorporate both Cy3- and Cy5-dCTPs with comparable efficiency. Synthesis through the G₈ template stretch was detected by incorporation of Digoxigenin-11-dUTP.(34) (b) PAGE gel of a 0.4kb PCR amplification (70% GC) with complete replacement of dCTP with either Cy5- or Cy3-dCTP comparing selected clones to wild-type Pfuexo- (Pfu). Only E10 (and to a lesser extent A3) are able to perform PCR amplification with both dyes. Visible spectrum absorption by the 287 cyanine dyes incorporated (on both strands) colors the DNA fragment brightly pink (Cy3) or blue (Cy5).