Figure 4.

(a) Organic phase partitioning of CyDNA is shown for Cy3-DNA (left) and Cy5-DNA (right). Essentially 100% partioning occurs in the presence of 150 mM NaCl (the yellow color of the phenol phase is due to addition of 8-hydroxyquinoline to prevent oxidation). (b) Agarose gel electrophoresis of CyDNA restriction digests. Both Cy3- and Cy5-DNA are resistant to cleavage by the restriction endonuclease DdeI but are cut by MseI. Cy5-DNA displays no fluorescence despite ethidium bromide staining, while Cy3-DNA fluorescence is due to UV excitation (and seen to the same extent in ethidium bromide free gels (not shown)). m is ϕX HaeIII marker.