Table 1. Polymerase Fidelity.
| polymerase | PCR substrates | mutations/kb | mutation rate/bp/doublinga |
|---|---|---|---|
| Pfuexo- | dNTPs | 1.1 | 4.4 × 10−5 |
| (4.7 × 10−5)b | |||
| E10 | dNTPs | 0.4 | 1.6 × 10−5 |
| (2.6 without additivesc) | (1.04 × 10−4)c | ||
| Pfuexo- | dNTPsb | 2.7 | 6.0 × 10−5 |
| E10 | dATP, dGTP, dTTP, Cy3-dCTP | 4.3 | 9.6 × 10−5 |
| E10 | dATP, dGTP, dTTP, Cy5-dCTP | 4.9 | 1.1 × 10−4 |
a
Corrected for the number of doublings (PCR cycles).
b
As determined by a lacZ reversion assay.(44)
c
In the absence of additives (1% formamide, 10% glycerol, 10 μg/mL RNase, 1 mM DTT).