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. Author manuscript; available in PMC: 2010 May 1.
Published in final edited form as: J Immunol. 2009 May 1;182(9):5537–5546. doi: 10.4049/jimmunol.0803742

FIGURE 5.

FIGURE 5

Safety studies using the anti-angiogenesis Flk-1 vaccines. Mice were immunized thrice, as performed in all previous experiments, and then were allowed to either mate or entered into wound-healing studies. A, Mice (n = 5/group) were mated with syngeneic FVB/N males, and gestation was confirmed upon the observance of a vaginal plug following coitus. This was considered as day 0.5 days post-coitus. Total gestation length, pup mass at term, and total litter size were measured; graphs show mean ± SEM; *, p < 0.05. B, A pair of sterile skin biopsies was produced on the back of each vaccinated mouse (n = 5/group). Healing was observed on a daily basis. On day 14, healing was complete for all groups tested; near identical healing was observed for all groups. Graph shows the number of days until wound closure, mean ± SEM; *, p < 0.05; Mann-Whitney U test.