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. Author manuscript; available in PMC: 2010 May 1.
Published in final edited form as: J Immunol. 2009 May 1;182(9):5537–5546. doi: 10.4049/jimmunol.0803742

FIGURE 6.

FIGURE 6

Flk-1 vaccine-induced epitope spreading may not be due to cross-reactivity between Flk-1 and Her-2/neu shared domains. Mice were immunized thrice with either control Lm or Flk-I1 vaccine. Splenocytes were processed and rechallenged ex vivo for the secretion of IFN-γ in response to peptide challenge. Peptides included were the previously mapped pFlk-I1 epitope (PGGPLMVIV), a putative pIC1 epitope for Her-2/neu (GSGAFGTVYK) or the epitope in question, a putative shared epitope between the Her-2/neu and Flk-1 kinase domains (GRGAFGQVI), and a third-party epitope used as a negative control (pGag). Graph shows mean ± SEM; n = 3/group.