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. Author manuscript; available in PMC: 2010 Apr 7.
Published in final edited form as: Mol Cell Biomech. 2009;7(2):77–91.

Figure 3. Effects of shear stress and shear rate on melanoma adhesion to the EC in the presence of PMNs.

Figure 3

The parallel plate flow chamber was perfused with appropriate medium over the EI monolayer for 2–3 minutes at a shear rate of 40 sec−1 for equilibration and then PMNs and WM9 cells were injected into the flow chamber through the syringe pump at a predetermined concentration (1×106 cells/ml) of each. The cell-cell interactions in shear flow were then recorded and analyzed off-line. WM9 adhesion efficiency was defined in “Materials and Methods”. Values are mean ± S.E.M. for N ≥ 3.