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. Author manuscript; available in PMC: 2010 Apr 7.
Published in final edited form as: Biochemistry. 2010 Feb 2;49(4):782–792. doi: 10.1021/bi901446r

Figure 3. Equilibrium binding of the alkaline phosphatase signal peptide to SecA as determined by fluorescence anisotropy.

Figure 3

Binding of IANBD-labeled SP22 (■)or SP2 (●) to SecA was assessed by fluorescence anisotropy (r). Signal peptides were maintained at a constant concentration of 1 μM, and wild-type SecA was titrated into the system from 0 to 40 μM. All experiments were performed in TKE buffer at 20 °C.