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. Author manuscript; available in PMC: 2010 Apr 7.
Published in final edited form as: Biochemistry. 2010 Feb 2;49(4):782–792. doi: 10.1021/bi901446r

Figure 4. Stoichiometry of SecA–signal peptide binding as determined by fluorescence anisotropy.

Figure 4

Binding of IANBD-labeled SP22 (■) to SecA was assessed as fluorescence anisotropy (r) and plotted to reveal the saturated molar ratio of SP22 to SecA protomer. IANBD-labeled SP22 was titrated from 0 to 20 μM into a solution of 14 μM wild-type SecA. All experiments were performed in TKE buffer at 20 °C.