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. Author manuscript; available in PMC: 2010 Apr 7.
Published in final edited form as: Biochemistry. 2010 Feb 2;49(4):782–792. doi: 10.1021/bi901446r

Figure 5. Equilibrium binding of SP22 to monomeric SecA.

Figure 5

Binding of IANBD-labeled SP22 to IAEDANS-labeled SecAΔ11-Cys-190 (■) was assessed by FRET. SecAΔ11-Cys-190 was maintained at a constant concentration of 0.05 μM, and SP22 was titrated into the system from 0 to 15 μM. All experiments were performed in TKE buffer at 20 °C.