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. Author manuscript; available in PMC: 2010 Apr 7.
Published in final edited form as: Science. 2009 Apr 3;324(5923):105–108. doi: 10.1126/science.1166657

Fig. 4.

Fig. 4

LNV synapse number in medulla. (A and B) LNV projections in socially enriched pdf-GAL4/+;;UAS-dlgWT-gfp/+ flies contain more GFP-positive terminals than their socially isolated siblings when evaluated using confocal microscopy. (C) Relative quantification of dlg-GFP-immunopositive terminals in socially isolated pdf-GAL4/+;;UAS-dlgWT-gfp/+ flies versus socially enriched siblings (P = 1.5×10−; n =33 to 34 in each group). (D) dlg-GFP positive terminals 48 hours after social enrichment in sleep-deprived flies and their normally sleeping siblings. (P = 0.003; n = 15 to 18 in each group). (E and F) Social enrichment of pdf-GAL4/+;UAS-VAMP-GFP/+ flies induces a modest increase of LNV terminals relative to isolated siblings. (G) Relative quantification of VAMP-GFP-positive terminals in socially isolated pdf-GAL4/+;UAS-VAMP-gfp/+ flies and their socially enriched siblings (P = 0.03; n =16 to 18 in each group).(H) UAS-VAMP-gfp-positive terminals 48 hours after social enrichment in sleep-deprived flies and their normally sleeping siblings (P = 0.01; n = 18 in each group).