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. Author manuscript; available in PMC: 2010 Apr 7.
Published in final edited form as: Methods Mol Biol. 2009;582:49–57. doi: 10.1007/978-1-60761-340-4_5

Fig. 1.

Fig. 1

Gel shift assay with topo70 Y/F and topo31. Equal amounts of supercoiled circles (SC), linears (L) and nicked circles (NC) of pKSII+ plasmid DNA (0.08 pmol each) were incubated with increasing amounts of the indicated proteins in 20 μl of DNA binding buffer and analyzed by agarose gel electrophoresis as described in the text. Lanes 1, 7 and 13 contained no protein to mark the mobilities of the unbound DNAs. Lanes 2-6 contained 0.88, 1.75, 3.5, 7 and 15 pmol of topo70 Y/F protein, respectively. Lanes 8-12 contained 1.75, 3.5, 7, 15, and 30 pmol of topo31, respectively.