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. Author manuscript; available in PMC: 2010 Apr 7.
Published in final edited form as: Methods Mol Biol. 2009;582:49–57. doi: 10.1007/978-1-60761-340-4_5

Fig. 2.

Fig. 2

Filter binding assay. 3H-labeled nicked SV40 DNA (0.2 pmol) was incubated with topo70 Y/F and then challenged with increasing amounts of unlabeled nicked competitor (filled diamonds) or unlabeled supercoiled competitor (open diamonds) as described in the text. The percentage of 3H-labeled nicked SV40 DNA retained on the filter is plotted against the ratio of the unlabeled competitor to labeled DNA.