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. Author manuscript; available in PMC: 2010 Apr 7.
Published in final edited form as: Biochemistry. 2009 Jun 9;48(22):5007–5017. doi: 10.1021/bi900196c

Figure 7.

Figure 7

H2A.Z can make a heteromorphic nucleosome with H2A. (A) Scheme for making the heteromorphic nucleosomes. (B) Native PAGE of the reconstituted sucrose gradient-purified nucleosomes loaded onto the Ni+ column. M: CfoI-digested pBR322 plasmid DNA as a marker. A: H2A nucleosome. Z: H2A. Z nucleosome. H: H2A–H2A.Z heteromorphic nucleosome. (C) SDS–PAGE (15%) analysis of nucleosomes after they had passed through the Ni+ column. M: chicken erythrocyte histone marker. B: nucleosomes before loading onto the Ni+ column. W: wash fractions. E: eluted fractions. Mix: H2A–H2A.Z heteromorphic nucleosome.