Skip to main content
. Author manuscript; available in PMC: 2010 Oct 1.
Published in final edited form as: Nat Methods. 2010 Mar 7;7(4):307–309. doi: 10.1038/nmeth.1438

Figure 2.

Figure 2

SeCreEt parasites activate eGFP in reporter cell lines. (a) Schematic of the retroviral construct used to produce the Cre-reporter cell line. The DsRed and eGFP coding regions are driven by a promoter in the long terminal repeat (LTR) but have three stop codons separating them, thus preventing expression of eGFP. Arrow indicates transcription start. Blue triangles indicated loxP sites and their orientation. SD is splice donor, SA is splice acceptor, and Psi (ψ) is a retroviral packaging signal. (b,c) Cre-reporter cells 24 hours after infection with parental strain (b) or SeCreEt parasites (c). Panels from left to right: phase, red shows both DsRed expression and staining with Toxoplasma-specific anti-SAG1 antibody, green shows eGFP expression, merge. Scale bars, 5 μm. (d) The percentage of SeCreEt parasite-infected Cre-reporter cells that showed eGFP expression is plotted at the indicated time points.