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. Author manuscript; available in PMC: 2010 Apr 7.
Published in final edited form as: Methods Mol Biol. 2009;500:335–359. doi: 10.1007/978-1-59745-525-1_12
1 function signal = eq_binding(Et, Lt, Kd, signal, gain, c)
2 %binding equilibrium E + L < = > EL; simulate signal proportional to [EL]
3 %<gfitModelDescription version=“100”>
4 %variable type minVal startVal unit comment
5 % Et () 0 1 uM ‘total concentration of E’
6 % Lt independent 0 1 uM ‘total concentration of L’
7 % Kd para 0 0.1 uM ‘dissociation constant’
8 % signal dependent () () () ‘complex formation’
9 % gain para () 1 () ‘signal gain’
10 % c para () 0 () ‘signal background’
11 %
12 %variable size plotVs
13 % Et Lt ()
14 % signal Lt Lt
15 %</gfitModelDescription>
16 EL = (Kd + Et + Lt - sqrt((Kd + Et + Lt). ˆ2 - 4 * Et.* Lt))/2;
17 signal = c + gain * EL;