Figure 2.

Cysteine 95 and other residues dampened the inhibitory effect of H69E on protease autoprocessing in transfected mammalian cells. (A) The indicated proviral DNAs were transfected into HEK 293T cells grown on 6-well plates with calcium phosphate. The total cell lysates and VLPs were prepared as described (Material and Methods) and subjected to western blot analysis. Mouse monoclonal anti-p24 antibody was used to detect proteins such as the full length Gag polyprotein (p55), CA-p2 intermediate (p25), and final processing product (p24) in the transfected cells and the released VLPs. The cell lysates blot was stripped and reprobed for GAPDH as loading controls. (B) Relative Gag processing efficiencies were quantified from three independent experiments and the bars represent standard deviations.