Figure 1. Western blot analysis showing specificity of antisera that recognize Drosophila dMfn, Opa1, and Drp1.

(A) Protein extracts from wt flies and flies expressing either the UAS-dmfn-RNAi^Vienna or UAS-dmfn-RNAi^Guo transgenes targeting the dmfn gene were subjected to western blot analysis with an anti-dMfn antiserum. The hsp70-GAL4 driver was used to induce expression of the dmfn-RNAi transgenes, and flies were collected for analysis 24 to 48 hrs after a 3-hr heat shock protocol. The arrow indicates the location of the dMfn band at 94 kDa. The asterisks (*) indicate two nonspecific bands detected at 80 kDa and 110 kDa. (B) Protein extracts from wt flies, flies bearing the UAS-Opa1-FLAG transgene and the mesoderm-specific 24B-GAL4 driver, and flies bearing the UAS-opa1RNAi transgene and the muscle-specific dmef2-GAL4 driver were subjected to western blot analysis with an anti-Opa1 antiserum. (C) Protein extracts from wt flies, flies bearing the UAS-Drp1-HA transgene and the muscle-specific dmef2-GAL4 driver, and flies heterozygous for the Df(2L)Excel6008 deletion chromosome (which removes the drp1 gene) were subjected to western blot analysis with an affinity-purified anti-Drp1 antiserum.