. Author manuscript; available in PMC: 2010 Apr 8.

Published in final edited form as: Toxicol Appl Pharmacol. 2006 Jun 27;216(1):69–79. doi: 10.1016/j.taap.2006.04.011

Table 1.

Growth kinetics of UROtsa and URO-MSC cells after treatment with MMA^III

Cell line	Doubling time (h)
UROtsa	42 ± 1.9
URO-MSC, 12 weeks	27 ± 0.3^*
URO-MSC, 24 weeks	25 ± 0.2^*
URO-MSC, 52 weeks	21 ± 0.2^*

UROtsa were continuously exposed to 0.05 μM MMAIII for 12, 24, and 52 weeks. Trypan blue exclusion assay was used to enumerate cells at 24, 48, 72, and 96 h after plating. Then, growth curves were generated and used to estimate doubling times of treated and untreated cells. Data represent results from one experiment (n = 3). ANOVA followed by Bonferroni’s multiple comparisons test was used to identify significant changes in doubling time.

Significant deviations from control cells (P < 0.05) were marked with an asterisk.