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. Author manuscript; available in PMC: 2010 Oct 1.
Published in final edited form as: Biochem J. 2009 Sep 14;423(1):41–52. doi: 10.1042/BJ20090786

Figure 1. Anti-horseradish peroxidase epitopes of axenically-cultured Dicytostelium discoideum.

Figure 1

(A) The AX3 strain exhibits somewhat quicker growth during the pre-logarithmic phase of axenic culture in comparison to the HL250 (modC) strain; (B) Western blotting shows the presence of anti-HRP epitopes in axenically-grown AX3, but not in axenically-grown HL250, consistent with a defect in fucose metabolism in that strain; a partial recovery of anti-HRP epitopes was observed when HL250 cells were grown in the presence of exogenous fucose. The Coomassie-stained gel shows that protein loading was approximately equal. (C) Flow cytometry also shows the absence of anti-HRP epitopes in the HL250 strain grown in the absence of fucose (green) as compared to AX3 (red) or HL250 grown in the presence of exogenous fucose (blue).