. Author manuscript; available in PMC: 2010 Oct 1.

Published in final edited form as: Biochem J. 2009 Sep 14;423(1):41–52. doi: 10.1042/BJ20090786

Table 1. Linkage analysis of the Man₈GlcNAc₄ and Man₈GlcNAc₄Fuc₁ N-glycans.

Both the purified pyridylaminated H8N4 and H8N4F1 glycans derived from axenically-grown AX3 were subject to permethylation. Partially methylated alditol acetates, obtained after hydrolysis, reduction and peracetylation, were separated on an RTX200 column and analysed by GLC/MS in the positive-ion CI-mode. The innermost reducing terminal GlcNAc is not detected because of PA-labelling. The data obtained reveal the presence of terminal bisecting and intersecting GlcNAc in both cases, which is corroborated by the exclusive detection of 3,4,6-trisubstituted Man and the lack of 3,6-disubstituted Man. The results obtained were as follows:

Methylated sugar derivative	H₈N₄-PA	H₈N₄F-PA	Linkage
2,3,4-Fuc	−	(+)^a	Fuc(1-
2,3,4,6-Man	+	+	Man(1-
3,4,6-Man	+	+	-2)Man(1-
2,4-Man	−	−	-3,6)Man(1-
3,4-Man	−	−	-2,6)Man(1-
2,3-Man	−	−	-4,6)Man(1-
2-Man	+	+	-3,4,6)Man(1-
3,4,6-GlcNAc	+	+	GlcNAc(1-
3,6GlcNAc	+	(+)^a	-4)GlcNAc(1-

Trace amounts, due to low amount of material.

Table 1. Linkage analysis of the Man8GlcNAc4 and Man8GlcNAc4Fuc1 N-glycans.

Table 1. Linkage analysis of the Man₈GlcNAc₄ and Man₈GlcNAc₄Fuc₁ N-glycans.