Table 1.

Results from in vitro peptide array phosphorylation assays examining GluR1 phosphorylation at T840

Kinase	Protein	Sequence	Site	Level (%)
PKA	GluR1	NEAIRTSTLPRNSGA	T840	0
PKA	Control	LRRASLGVLKI	Consensus	100
Akt	GluR1	NEAIRTSTLPRNSGA	T840	0
Akt	Control	GVGRPRTSSFAEGAG	Consensus	100
p70S6K	GluR1	NEAIRTSTLPRNSGA	T840	38 ± 10
p70S6K	GluR1	NEAIRTSVLPRNSGA	T840V	0
p70S6K	Control	GGKKRNRTLTKKRGA	Consensus	100

Although PKA and Akt failed to phosphorylate the GluR1 T840 peptide, significant phosphorylation was detected in assays with p70S6K. Levels show optical density measurements normalized to signals from positive control peptides in the same array containing consensus sites for PKA, Akt, or p70S6K. Note that p70S6K phosphorylation of the peptide is abolished when T840 is replaced with a valine (T840V).