EPR spectra of NO formation in the heart from nitrite. Hearts were prelabeled with 1 mM 15N or 14N nitrite. The left panel shows spectra from hearts with Fe-MGD; A, before ischemia; B, 30 min of ischemia with 15N-nitrite; C, 30 min ischemia with 14N-nitrite. The right panel shows nitosyl-heme formation; A, with 14N-nitrite; B, with 15N-nitrite.