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. Author manuscript; available in PMC: 2011 Feb 15.

Published in final edited form as: Nitric Oxide. 2010 Jan 5;22(2):83–90. doi: 10.1016/j.niox.2009.12.004

Fig. 5 — Lineweaver-Burk analysis of NO generation by XO as a function of nitrite concentration at different oxygen tensions. Reciprocal plot, 1/velocity versus 1/[nitrite]. A, 0.02 mg/ml XO, 0.02 mM xanthine in the presence of 400 units/ml SOD and 4–60 mM nitrite. B, 0.02 mg/ml XO and 0.5 mM DBA in the presence of 400 units/ml SOD and 2–64 mM nitrite. Oxygen concentrations of 214, 102, 51, and 20 μM, respectively, were achieved with purging of the sample solutions at 37 °C with 21, 10, 5, or 2% oxygen. Reproduced from [18].

Fig. 5 — Lineweaver-Burk analysis of NO generation by XO as a function of nitrite concentration at different oxygen tensions. Reciprocal plot, 1/velocity versus 1/[nitrite]. A, 0.02 mg/ml XO, 0.02 mM xanthine in the presence of 400 units/ml SOD and 4–60 mM nitrite. B, 0.02 mg/ml XO and 0.5 mM DBA in the presence of 400 units/ml SOD and 2–64 mM nitrite. Oxygen concentrations of 214, 102, 51, and 20 μM, respectively, were achieved with purging of the sample solutions at 37 °C with 21, 10, 5, or 2% oxygen. Reproduced from [18].