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. Author manuscript; available in PMC: 2010 Apr 8.
Published in final edited form as: Dev Biol. 2009 Jan 20;328(1):13–23. doi: 10.1016/j.ydbio.2008.12.041

Fig. 1. Twsg1 gene expression and protein localization in the mandibular component of BA1.

Fig. 1

(A-F) Whole mount LacZ staining of heterozygous embryos carrying LacZ reporter gene in the Twsg1 locus. (A) Negative control at E9.5. (B) LacZ reporter gene is expressed throughout the mandibular arch at E9.5, in both distal and proximal regions. (C) Negative control at E10.5. (D) Gradient of the LacZ reporter gene expression at E10.5 with predominance in the distal region. (E) Negative control at E11.5. (F) The reporter gene is expressed in a pattern that is restricted toward the oral epithelium and proximal arch at E11.5. (G-O) TWSG1 protein localization by fluorescent immunostaining (right arch is shown). (G) DAPI, higher magnification of the image shown in M, (H) TWSG1 protein, (I) Overlay of DAPI and immunostaining for TWSG1 showing localization to the cell membranes, (J) DAPI, (K) Overlay of DAPI and TWSG1 immunostaining showing uniform distribution of TWSG1 throughout the mesenchyme of the mandibular arch at E9.5, (L) DAPI, (M) Overlay of DAPI and TWSG1 at E10.5 showing a gradient of distribution of TWSG1, (N) DAPI, (O) Overlay of DAPI and TWSG1 at E11.5 showing a shift in TWSG1 protein distribution toward the proximal BA1. Sections G-O are transverse sections; d, distal; p, proximal. Scale bar: 200 μm in A-F, 10 μm in G-I, 50 μm in J-O.