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EPR spectra of the tyrosyl radical formed during reaction of Mn-PGHS-1 with 15-HPETE. Mn-PGHS-1 samples (40 µM) in 100 mM KPi (pH 7.2), 0.04% octyl glucoside, and 10% glycerol were reacted at room temperature with 2 equiv of 15-HPETE for the indicated times before freeze trapping. The arrow indicates the direction of intensity changes with time. EPR conditions are the same as those described in the legend of Figure 2.
