FIGURE 9.

Comparison of observed kinetics of A₄₂₈ (●) and normalized remaining peroxidase activity (□) during reaction of Fe-PGHS-1 with 20 equiv of EtOOH (A) or 15-HPETE (B) from the experiments in Figure 6 and Figure 7 with computer simulations (─) generated as described in Experimental Procedures. The optimal values of rate constants for reaction with EtOOH are listed in Table 1, and the following parameters were used: ε1 = 0.063 µM⁻¹ cm⁻¹, ε2 = 0.102 µM⁻¹ cm⁻¹, ε3 = 0.080 µM⁻¹ cm⁻¹, ε4 = 0.061 µM⁻¹ cm⁻¹, and Residual = 0.34. The optimal values of rate constants for reaction with 15-HPETE are listed in Table 1, and the following parameters were used: ε1 = 0.060 µM⁻¹ cm⁻¹, ε2 = 0.091 µM⁻¹ cm⁻¹, ε3 = 0.068 µM⁻¹ cm⁻¹, ε4 = 0.049 µM⁻¹ cm⁻¹, and Residual = 0.05.