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. 2010 Feb 4;151(4):1929–1939. doi: 10.1210/en.2009-1017

Figure 5.

Figure 5

Activation of p38 MAPK signaling pathway in the thyroid of TRβPV/PV mice but not in WT-PTU mice. A, Thyroid extract (30 μg) was used in the Western blot analysis, as described in Materials and Methods. The effectors analyzed in the p38 MAPK phosphorylation cascade are marked. Two representative results from five to seven WT (lanes 1 and 2), TRβPV/PV (lanes 3 and 4), and WT-PTU (lanes 5 and 6) mice are shown. B, Increased expression of MMP-9 in TRβPV/PV mice, but not in WT-PTU mice, at the protein level (a) as well as the mRNA level (b). C, Increased protein levels of TGFβ and TGFβR1 in thyroids of WT, TRβPV/PV, and WT-PTU mice determined by Western blot analysis. Thirty micrograms of thyroid extract were used and two representative results from four to six WT (lanes 1 and 2), TRβPV/PV (lanes 3 and 4), and WT-PTU (lanes 5 and 6) mice are shown. D, The expression of TGFβ gene (Tgfb1) mRNA was not significantly different in thyroids of WT, TRβPV/PV, and WT-PTU mice. Total RNA from five to six mouse thyroids per each group was analyzed by RT-PCR and results are shown as fold change to that of WT mice. N.S., Not significant.