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. Author manuscript; available in PMC: 2010 Apr 8.
Published in final edited form as: Biochemistry. 2006 Jan 17;45(2):523–532. doi: 10.1021/bi051235w

Figure 4.

Figure 4

EPR spectra of radicals formed during anaerobic reaction of Y348F/Y504F PGHS-2 with AA or d8-AA. (A) Reaction at 0 °C of Y348F/Y504F PGHS-2 (15 μM heme) with 15 equiv of EtOOH for 26 s and then with 4 equiv of AA for 11 s (—). The simulated EPR spectrum (– – –) is an arithmetic combination of 59% Y348F/Y504F PGHS-2 NS and 41% AA-pentadienyl radical. (B) Reaction at 0 °C of Y348F/Y504F PGHS-2 (15 μM heme) with 15 equiv of EtOOH for 19 s and then with 4 equiv of d8-AA for 13 s (—). The simulated EPR spectrum (– – –) is an arithmetic combination of 54% Y348F/Y504F PGHS-2 NS and 46% d8-AA-pentadienyl radical. Simulation parameters are given in Table 3 and ref 38.