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. Author manuscript; available in PMC: 2010 Apr 8.
Published in final edited form as: Biochemistry. 2006 Jan 17;45(2):523–532. doi: 10.1021/bi051235w

Table 2.

Kinetic Parameters for Cyclooxygenase Inhibition by Nimesulidea

PGHS-2 construct Ki (μM) k2 (min−1) k−2 (min−1)
wild-type 1.0 ± 0.2 2.2 ± 0.3 0.29 ± 0.02
Y504F 0.9 ± 0.2 2.0 ± 0.2 0.73 ± 0.05
Y348F/Y504F 0.27 ± 0.06 2.8 ± 0.5 0.43 ± 0.03
a

Data from the experiments shown in Figure 5 were analyzed as described in Experimental Procedures.