Skip to main content
. Author manuscript; available in PMC: 2010 Apr 8.
Published in final edited form as: J Biomol Screen. 2008 Jan;13(1):62–71. doi: 10.1177/1087057107312127

Figure 4.

Figure 4

Effect of cAMP in the preculture on rolipram- or cAMP-stimulated 5FOA-resistant growth. Strain CHP1113 (PDE4B) cells were precultured in EMM medium containing various concentrations of cAMP as indicated. Cells were then transferred to 5FOA medium containing DMSO (white bars, vehicle control), 20μM rolipram (black bars), or 5mM cAMP (gray bars) and incubated at 30°C for 48 hours. OD600 values are normalized to the DMSO controls. Cells precultured in less than 0.25mM cAMP fail to respond either rolipram or cAMP.