Table 2.

Kinetic constants of the formation and decay of the oxyferrous complexes for the reaction of oxygen with various ferrous P450s^a. Temp., temperature.

	kon (M−1·s−1)	koff (s−1)	Kd (µM)	kdecay (s−1)	Temp. (°C)
PGIS (this study)	5.9 × 105	29	49	12	4
P4503A4 [31]
+ testosterone	5.0 × 105			0.37	6
− testosterone				20	5
Rhizobium [38]
P450a	5 × 105			2.2	4
P450b	7 × 105			1.6	4
P450c	7 × 105			4.8	4
Pseudomonas putida P450cam [4,34]
+ camphor	7.7 × 105 (4 °C)		1.4	0.55 × 10−3	2
− camphor	Very fast			1.8 × 10−3	2
Adrenal cortex mitochondria P450scc [36,41,42]
+ cholesterol	3.8 × 105	4.7	12	6.3	4
+ cholesterol	1.3 × 106 (8 °C)		23	6.1 × 10−3	2
− cholesterol	Very fast			4.0 × 10−3	−17
Hepatic microsomes [37,43,44]
P450LM2	4.4 × 106 (25 °C)			3.7	2
P450LM3	~ 106 (25 °C)			4.3	10
P450LM4	5.0 × 105		0.7	0.9	10
Caldariomyces fumago [40]
Chloroperoxidase (pH 2.8–6.8)	5.5 × 105	8–32	10–70		25
Nitric oxide synthase [32]
+ arginine	4 × 105	5.5	13.8	1.4	4
− arginine	1.4 × 105	60	43	5–30	4

^aUnless otherwise indicated, the experiments were carried out at a pH of 7.1–7.5.