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. 1971 May;106(2):493–499. doi: 10.1128/jb.106.2.493-499.1971

Valine Accumulation by α-Aminobutyric Acid-Resistant Mutants of Serratia marcescens

Masahiko Kisumi 1, Saburo Komatsubara 1, Ichiro Chibata 1
PMCID: PMC285121  PMID: 4929861

Abstract

α-Aminobutyric acid, norvaline, and norleucine, which are analogues of branched-chain amino acids, inhibited the growth of Serratia marcescens. The inhibitory effect of these three analogues was counteracted by branched-chain amino acids. A number of mutants resistant to these analogues were isolated. α-Aminobutyric acid-resistant (abu-r) mutants markedly accumulated l-valine in the culture medium, but the other analogue-resistant mutants did not. Acetohydroxy acid synthetase, which seems to be rate-limiting for the biosynthesis of l-valine, was derepressed in abu-r mutants. One of the abu-r mutants, no. 140, which accumulated over 8 mg of l-valine per ml, had about a 20-fold increase in the enzyme level. Most of the abu-r mutants had acetohydroxy acid synthetase activity which was sensitive to feedback inhibition by l-valine to the same extent as in the parent strain. However, the enzyme of two of abu-r mutants was less sensitive to l-valine, and one of the two was the best valine accumulator.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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