Figure 1.
Evidence of IL-7 receptor expression on human eosinophils. A, Representative immunoblot analysis for CD127 in cell lysates from highly purified (>99%) eosinophils (Eos) or CD4+ T lymphocytes (CD4) immunoblotted with either a monoclonal antibody specific to CD127 (left panel) or an isotype control IgG1 (right panel). B, CD127 expression was analyzed by immunoblotting of 30 µg of cell lysate protein from 7 separate eosinophil donors and quantified by densitometry. For each donor, the immunoblot density is summarized as the % of CD127 signal from 30 µg of the positive control T lymphocyte cell lysate run on the same gel and immunoblot.