Table 1.

Primer sequences of human genes examined by quantitative real-time PCR. AS, antisense; COX-2, cyclooxygenase-2; HPRT, hypoxanthine guanine phosphoribosyltransferase; IFN-β, interferon-β; IL-1β, interleukin-1β; IL-6, interleukin-6; IL-8, interleukin-8; IP-10, 10 kDa interferon-γ-induced protein; MCP-1, monocyte chemotactic protein-1; MIP-2α, macrophage inflammatory protein-2α; MIP-3α, macrophage inflammatory protein-3α; RANTES, regulated upon activation, normal T-cell expressed, and secreted; S, sense; TNF-α, tumor necrosis factor-α.

Target gene	Primer sequence
HPRT	5’-CAAGCTTGCTGGTGAAAAGGAC-3’ (S) 5’-GTCAAGGGCATATCCTACAACAAA-3’ (AS)
IL-8	5’-ATAAAGACATACTCCAAACCTTTCCAC-3’ (S) 5’-AAGCTTTACAATAATTTCTGTGTTGGC-3’ (AS)
MIP-3α	5’-GCGGCGAATCAGAAGCA-3’ (S) 5’-GGCCAGCTGCCGTGTG-3’ (AS)
IFN-β	5’-GGCAATTGAATGGGAGGCT-3’ (S) 5’-GGCGTCCTCCTTCTGGAACT-3’ (AS)
IP-10	5’-TGACTCTAAGTGGCATTCAAGGAG-3’ (S) 5’-TTTTTCTAAAGACCTTGGATTAACAGG-3’ (AS)
RANTES	5’-TTTGCCTACATTGCCCGC-3’ (S) 5’-TTTCGGGTGACAAAGACGACT-3’ (AS)
TNF-α	5’-CCCAGGGACCTCTCTCTAATCA-3’ (S) 5’-GCTTGAGGGTTTGCTACAACATG-3’ (AS)
IL-6	5’-GTAGCCGCCCCACACAGA-3’ (S) 5’-CATGTCTCCTTTCTCAGGGCTG-3’ (AS)
IL-1β	5’-AAATACCTGTGGCCTTGGGC-3’ (S) 5’-TTTGGGATCTACACTCTCCAGCT-3’ (AS)
MIP-2α	5’-CGCCCAAACCGAAGTCAT-3’ (S) 5’-GATTTGCCATTTTTCAGCATCTTT-3’ (AS)
MCP-1	5’-ACTCTCGCCTCCAGCATGAA-3’ (S) 5’-TTGATTGCATCTGGCTGAGC-3’ (AS)
COX-2	5’-CCCATGTCAAAACCGAGGTG-3’ (S) 5’-CCGGTGTTGAGCAGTTTTCTC-3’ (AS)