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. Author manuscript; available in PMC: 2011 Apr 13.
Published in final edited form as: Biochemistry. 2010 Apr 13;49(14):3148–3155. doi: 10.1021/bi902189s

FIGURE 6.

FIGURE 6

TRBP recognition of 21 nt single-stranded RNAs. The ssRNAs from each siRNA duplex were tested by gel shift assay for (A) crosslinking, (B) native binding, or (C) native loading control, and (D) the fraction of siRNA crosslinked by MBP-TRBP was quantified within each lane, as in Figure 4. Values are average ± standard deviation; n ≥4. Note the break in the ordinate indicated by the cross-hatch. The ‘Fold Diff.’ values listed below the graph are the ratios between the AS fraction crosslinked and the SS fraction crosslinked. Symbols indicate statistically significant difference for: *, p < 1×10−3 and #, p < 2×10−2.