Figure 2.

Effects of pre-incubating PBMCs with various concentrations (0–30 µM) of unlabelled lopinavir (LPV) followed by the addition of [¹⁴C]LPV and co-incubating the cells with [¹⁴C]LPV followed by various concentrations (0–30 µM) of unlabelled LPV (trans-stimulation) on the accumulation of [¹⁴C]LPV. Bars indicate mean ± SD (n = 6, with four independent observations from each buffy coat PBMC sample). P values of *P < 0.05, **P < 0.01 and ***P < 0.001 indicate statistically significant differences in the CAR of [¹⁴C]LPV between control and LPV-treated samples.